Overexpression of Fusarium solani lipase in Pichia pastoris and its application in lipid degradation. Issue 5 (2nd September 2016)

• Record Type: Journal Article
• Authors: Wongwatanapaiboon, Jinaporn; Malilas, Waraporn; Ruangchainikom, Chalermchai; Thummadetsak, Gamgarn; Chulalaksananukul, Suphang; Marty, Alain; Chulalaksananukul, Warawut
• Is Part Of: Biotechnology, biotechnological equipment. Volume 30:Issue 5(2016)
• Page Start: 885
• Page End: 893
• Publication Date: 2016-09-02
• Subjects: Expression -- Fusarium solani -- lipase -- lipid degradation -- Pichia pastoris; Biotechnology -- Periodicals; Biotechnology -- Periodicals; Biotechnology -- instrumentation -- Periodicals; Periodicals; 660.605
• DOI: 10.1080/13102818.2016.1202779
• Languages: English
• ISSNs: 1310-2818

Abstract

ABSTRACT: Fusarium solani NAN103 lipase was successfully overexpressed in Pichia pastoris using inducible expression system and constitutive expression system under the control of alcohol oxidase 1 promoter (p AOX1 ) and glyceraldehyde-3-phosphate dehydrogenase promoter (p GAP ), respectively. Lipase obtained using the constitutive promoter showed the highest activity of 18.8 U/mg in 3 days of cultivation time. Optimal lipase activity was observed at pH 7.0 and 35 °C using p -nitrophenyl laurate as the substrate. Lipase activity was enhanced by Mn 2+, Ba 2+, Li +, Ca 2+, Ni 2+, CHAPS and Triton X-100 but was inhibited by Hg 2+, Ag + and SDS. The addition of 10% v/v of octanol, p -xylene, hexane and isopropanol increased lipase activity. Cultivation of lipase-expressing P. pastoris under p GAP in synthetic wastewater containing 1% w/v palm oil resulted in degradation of 87% of the oil within 72 h. P. pastoris expressing F. solani lipase from constitutive expression system has the potential to be used as an alternative microorganism for lipid degradation.