Simple biopsy modification to collect muscle samples from free-swimming sharks. (December 2018)

• Record Type: Journal Article
• Authors: Meyer, Lauren; Fox, Andrew; Huveneers, Charlie
• Is Part Of: Biological conservation. Volume 228(2018)
• Page Start: 142
• Page End: 147
• Publication Date: 2018-12
• Subjects: White shark -- Stable isotope -- Fatty acid -- Non-lethal sampling -- Tissue retention -- Conservation; Conservation of natural resources -- Periodicals; Nature conservation -- Periodicals; Ecology -- Periodicals; Environment -- Periodicals; Environmental Pollution -- Periodicals; Electronic journals; 333.9516
• DOI: 10.1016/j.biocon.2018.10.024
• Languages: English
• ISSNs: 0006-3207

Abstract

Abstract: Developing and enhancing non-lethal methods for sampling species of high conservation concern, including marine megafauna, has prompted the development of numerous biopsy methods to collect tissue for biochemical analyses. However, many of these analyses require adequately-sized muscle cores for reliable results. Here, we developed and trialed a novel modification to a biopsy probe traditionally limited to underwater use, which enables sampling of free-swimming sharks from above the surface. The modified probe used collected similar amounts of white shark, Carcharodon carcharias, muscle and sub-dermal tissue above water as the traditional underwater probe (muscle: 0.36 g vs. 0.44 g; sub-dermal tissue: 0.62 g vs. 0.44 g for surface and underwater respectively). Both methods obtained sufficient tissue for several analyses to be run on the same tissue core (e.g. stable isotopes, fatty acids, and genetics). This encourages the use of this biopsy probe, with studies assessing stock structure, trophic ecology, or physiology. The described modification adapts the probe to allow above-water deployment, providing more opportunities for effective, non-lethal sampling of free-swimming sharks. Graphical abstract: Highlights: Obtaining sufficient muscle tissue from large sharks can be challenging. Using a water balloon to trap water in a biopsy probe enables use from the surface. With the balloon the probe retains similar biopsies from the surface and underwater. The tissue retained is ample for isotope, lipid, ecotoxicology and genetic research. Taking biopsies from the surface provides additional sampling opportunities.