Immunostimulatory effect of Zinc Phthalocyanine derivatives on macrophages based on the pro-inflammatory TNFα and IL1β cytokine production levels. (December 2018)

• Record Type: Journal Article
• Authors: Ayaz, Furkan; Yuzer, Abdulcelil; Ince, Mine
• Is Part Of: Toxicology in vitro. Volume 53(2018)
• Page Start: 172
• Page End: 177
• Publication Date: 2018-12
• Subjects: Macrophages -- Inflammation -- Immunotoxicology -- PDT -- Phthalocyanine -- Adjuvants; TNFα Tumor necrosis factor α -- IL1β Interleukin 1 β -- IL6 Interleukin 6 -- LPS lipopolysachharide -- ELISA Enzyme linked immunosorbent assay -- PDT photodynamic therapy -- ZnPc Zinc Phthalocyanine; Toxicity testing -- In vitro -- Periodicals; Toxicology -- Periodicals; 615.9
• DOI: 10.1016/j.tiv.2018.08.011
• Languages: English
• ISSNs: 0887-2333

Abstract

Abstract: In this study, we examined the effect of halogen substitution on the ability of ZnPcs to stimulate or regulate the immune system cells. There have been studies focusing on the usage of Pcs as treatment option against different cancer types. More attention should be paid on their possible positive or negative effects on the immune system for better prognosis rates. We designed and synthesized unique ZnPcs with iodine substitution and further tested their effect on mammalian macrophage cell line. Macrophages are crucial cell types that can define the immune response by cytokine production as well as by antigen presentation to the other immune system cells after phagocytosis of the danger associated molecules. Our results suggest an immunostimulatory role for the iodine substituted ZnPc on macrophages based on the changes in pro-inflammatory cytokine production levels (TNFα, IL1β and IL6). This effect dependent on the presence of iodine; since non-substituted ZnPc did not exert such a stimulatory activity on the macrophages. These results support a possible use of the reagents against the tumor types that would get affected detrimentally by the pro-inflammatory environment. Graphical abstract: Highlights: Iodine substituted ZnPc stimulated TNFα, IL1β and IL6 production by macrophages. Iodine unsubstituted ZnPcs could not stimulate the macrophages by themselves. Therefore, the immunostimulatory effect relied on the Iodine substitution. ZnPcs did not affect TNFα and IL1β production by LPS stimulated macrophages. Whereas ZnPcs decreased the IL6 production by LPS stimulated macrophages.