Establishment of a new teleost brain cell line (DLB-1) from the European sea bass and its use to study metal toxicology. (February 2017)

• Record Type: Journal Article
• Authors: Morcillo, Patricia; Chaves-Pozo, Elena; Meseguer, José; Esteban, M. Ángeles; Cuesta, Alberto
• Is Part Of: Toxicology in vitro. Volume 38(2017)
• Page Start: 91
• Page End: 100
• Publication Date: 2017-02
• Subjects: European sea bass -- DLB-1 cell line -- Metals -- Apoptosis -- Oxidative stress; Toxicity testing -- In vitro -- Periodicals; Toxicology -- Periodicals; 615.9
• DOI: 10.1016/j.tiv.2016.10.005
• Languages: English
• ISSNs: 0887-2333

Abstract

Abstract: In teleost fish, there are no commercial cell lines for the European sea bass ( Dicentrarchus labrax ). Thus, we have established the sea bass brain (DLB-1) cell line, using a fish retrovirus for immortalization, which resemble epithelial cells and express glial cells markers. Exposure to metals [Cd, methylmercury (MeHg), Pb or As] produces cytotoxicity and induction of reactive oxygen species (ROS) production. Interestingly, cell cycle analysis of DLB-1 cells shows that exposure to metals alters it significantly. Moreover, all the metals induce apoptosis as indicated by sub-Go/G1 population and annexin V binding. Finally, exposure of DLB-1 cells to metals also produces significant alterations at gene expression level, which confirm the above functional results. This is the first study in which metal cytotoxicity has been evaluated in a fish brain cell line and results seem to support that DLB-1 cells are suitable for toxicological studies. Highlights: We have established a new brain cell line (DLB-1) from European sea bass. DLB-1 cells show rapid growth and glial origin. Metals produce cytotoxicity in DLB-1 being Cd the most toxic. DLB-1 cell line might be useful for fish neurotoxicological studies.