FTIR microscopy as a method for detection of retrovirally transformed cells. Issue 2 (2001)

• Record Type: Journal Article
• Authors: Huleihel Huleihel, Mahmoud Mahmoud; Talyshinsky Talyshinsky, Marina Marina; Erukhimovitch Erukhimovitch, Vitaly Vitaly
• Is Part Of: Spectroscopy. Volume 15:Issue 2(2001)
• Page Start: 57
• Page End: 64
• Publication Date: 2001
• DOI: 10.1155/2001/256362
• Languages: English
• ISSNs: 0712-4813

Abstract

Abstract : Fourier transform infrared (FTIR) spectroscopy is a unique technique that has potential for the optical diagnosis of cellular variations based on the characteristic molecular vibrational spectra of the cells. In the present study, a refinement of this technique − FTIR microscopy − was used in a mouse model to investigate spectral differences between primary cells and malignant cells transformed by murine sarcoma virus (MuSV). The advantage of FTIR microscopy is that it facilitates inspection of a restricted region of cell growth on a slide. A significant decrease in the intensities of the spectra was seen in malignant cells transformed by MuSV compared to primary normal cells. Also, there was a significant shift in the PO2 − symmetric stretching mode from 1082 cm −1 for normal cells to 1086 cm −1 for malignant cells. Detectable and consistent differences between normal primary and malignant cells were evident in the shapes and positions of a number of peaks in the FTIR spectra. Our results indicated that FTIR microscopy has potential as a diagnostic method for the detection of malignant cells.