Accessing the distance range of interest in biomolecules: Site-directed spin labeling and DEER spectroscopy. Issue 3 (2010)

• Record Type: Journal Article
• Authors: Böhme Böhme, Sabine Sabine; Steinhoff Steinhoff, Heinz-Jürgen Heinz-Jürgen; Klare Klare, Johann P. Johann P.
• Is Part Of: Spectroscopy. Volume 24:Issue 3/4(2010)
• Page Start: 283
• Page End: 288
• Publication Date: 2010
• Subjects: Site-directed spin labeling -- SDSL -- double electron–electron resonance -- DEER -- distance determination -- MTSSL -- spin label -- MnmE
• DOI: 10.3233/SPE-2010-0428
• Languages: English
• ISSNs: 0712-4813

Abstract

Abstract : Investigations on the structure and function of biomolecules often depend on the availability of topological information to build up structural models or to characterize conformational changes during function. Electron paramagnetic resonance (EPR) spectroscopy in combination with site–directed spin labeling (SDSL) allow to determine intra- and intermolecular distances in the range from 4–70 Å, covering the range of interest for biomolecules. The approach does not require crystalline samples and is well suited also for molecules exhibiting intrinsic flexibility. This article is intended to give an overview on pulsed EPR in conjunction with SDSL to study protein interactions as well as conformational changes, exemplified on the tRNA modifying enzyme MnmE.