The association of dimethylsulfoxide and model membranes studied by pulse-field gradient NMR. Issue 2 (2004)

• Record Type: Journal Article
• Authors: Kennedy Kennedy, Anthony Anthony; Long Long, Carla J. Carla J.; Hmel Hmel, Peter J. Peter J.; Hicks Hicks, Rickey Rickey; Reid Reid, Thomas J. Thomas J.
• Is Part Of: Spectroscopy. Volume 18:Issue 2(2004)
• Page Start: 265
• Page End: 269
• Publication Date: 2004
• DOI: 10.1155/2004/814213
• Languages: English
• ISSNs: 0712-4813

Abstract

Abstract : The use of dimethylsulfoxide (DMSO) as a cryoprotectant to reduce cellular injury during freezing is well known, however the intermolecular interactions between this amphiphilic molecule and biological membranes that form the basis of this protection are unknown. DMSO–dipalmitoylphosphatidylcholine (DPPC) vesicle interactions were investigated in pulsed-field gradient NMR (PFGNMR) experiments and spectra analysis allowed for the determination of self-diffusion coefficients for each species present. The mole fraction of DMSO associated with the DPPC vesicles was then calculated from the diffusion coefficients: the mole fraction increased from 14% to 42% as the membrane was heated from below to above the main phase transition temperature.