Thermodynamic and spectroscopic study on the binding of interaction anionic phthalocyanine with calf thymus DNA. Issue 6 (2011)

• Record Type: Journal Article
• Authors: Dezhampanah Dezhampanah, Hamid Hamid; Darvishzad Darvishzad, Termeh Termeh; Aghazadeh Aghazadeh, Mehrnaz Mehrnaz
• Is Part Of: Spectroscopy. Volume 26:Issue 6(2011)
• Page Start: 357
• Page End: 365
• Publication Date: 2011
• Subjects: DNA -- phthalocyanine -- thermodynamic of binding -- optical absorption -- SQUAD
• DOI: 10.3233/SPE-2012-0564
• Languages: English
• ISSNs: 0712-4813

Abstract

Abstract : The interaction between anionic form of copper (II) N, N', N", N'"-tetrasulfonated phthalocyanine Cu (tspc) and to calf thymus deoxyribonucleic acid (ct-DNA) is investigated by measuring UV-vis absorption and fluorescence spectroscopy in phosphate buffer. The binding constant and stoichiometry were determined by analysis of optical absorption spectra of phthalocyanine at various ct-DNA concentrations using SQUAD software. The static mode of fluorescence quenching of phthalocyanine by calf thymus deoxyribonucleic acid indicates the formation of a ground-state complex. The formation of ground-state complex is a spontaneous molecular interaction procedure in which outside groove binding through the formation of an axial bond between the base pairs of nucleotide and Cu in the central core of phthalocyanine.