A New Method for D2O/H2O Exchange in Infrared Spectroscopy of Proteins. Issue 5 (11th July 2012)

• Record Type: Journal Article
• Authors: Malferrari Malferrari, Marco Marco; Venturoli Venturoli, Giovanni Giovanni; Francia Francia, Francesco Francesco; Mezzetti Mezzetti, Alberto Alberto
• Is Part Of: Spectroscopy. Volume 27:Issue 5/6(2012)
• Page Start: 337
• Page End: 342
• Publication Date: 2012-07-11
• Subjects: D2O/H2O exchange -- photosynthetic reaction center -- FTIR spectroscopy -- isopiestic method -- protein hydration
• DOI: 10.1155/2012/791342
• Languages: English
• ISSNs: 0712-4813

Abstract

Abstract : In this paper, we describe a new method to obtain D2 O/H2 O exchange in photosynthetic reaction centres from Rhodobacter sphaeroides . The method is characterized by: (i) a very high efficiency of the isotopic replacement; (ii) an extremely low amount of D2 O needed; (iii) the short time required for dehydration and D2 O rehydration; (iv) the possibility of controlling concomitantly the hydration state of the sample. The proposed method can be applied to other proteins.