Affinity studies of hypocrellin B and mono-cysteine substituted hypocrellin B with CT-DNA using spectroscopic methods. Issue 5 (2005)

• Record Type: Journal Article
• Authors: Wei Wei, Shaohua Shaohua; Zhou Zhou, Jiahong Jiahong; Feng Feng, Yuying Yuying; Huang Huang, Deyin Deyin; Wang Wang, Xuesong Xuesong; Zhang Zhang, Baowen Baowen; Shen Shen, Jian Jian
• Is Part Of: Spectroscopy. Volume 19:Issue 5/6(2005)
• Page Start: 259
• Page End: 266
• Publication Date: 2005
• Subjects: Hypocrellin B -- MCHB, CT-DNA -- intercalation mechanism -- circular dichroism spectroscopy -- fluorescence spectroscopy -- anticancer drugs
• DOI: 10.1155/2005/258617
• Languages: English
• ISSNs: 0712-4813

Abstract

Abstract : The interaction of anticancer drug hypocrellin B (HB) and mono-cysteine substituted hypocrellin B (MCHB) with calf thymus deoxyribonucleic acid (CT-DNA) has been investigated using spectral methods. The results of UV–visible spectra showed that the HB and MCHB can intercalate into the base-stacking domain of the CT-DNA double helix. Further studies based on fluorescence spectroscopy and circular dichroism (CD) spectroscopy also supported the intercalation mechanism.