PS 13-01 AMELIORATION OF COGNITIVE IMPAIRMENT BY ADMINISTRATION OF BOVINE CASEIN-DERIVED PEPTIDE IN ALZHEIMER'S DISEASE MODEL MICE. (September 2016)

• Record Type: Journal Article
• Authors: Min, Li-Juan; Kobayashi, Yodai; Tsukuda, Kana; Yamada, Akio; Yamauchi, Koji; Abe, Fumiaki; Xiao, Jin-Zhong; Mogi, Masaki; Horiuchi, Masatsugu
• Is Part Of: Journal of hypertension. Volume 34:(2016) Supplement 1
• Publication Date: 2016-09
• Subjects: Hypertension -- Periodicals; Hypertension -- Periodicals; 616.132005
• DOI: 10.1097/01.hjh.0000501096.51933.aa
• Languages: English
• ISSNs: 1473-5598

Abstract

Abstract : Objective: There is a large interest in the prevention of dementia including Alzheimer's disease (AD) by natural food ingredients. Peptides derived from food proteins have been demonstrated to have hypotensive action. Increasing evidence indicated possible associations of hypertension with AD risk, and implied that angiotensin converting enzyme (ACE) inhibitors with the potential to pass blood-brain barrier may reduce the risk of AD. Here, we examined the effect of milk peptide (CH-3), which contains a tripeptide (methionine-lysine-proline, MKP) and has strong ACE inhibitory effect on cognitive function in AD model mice. Design and Method: Male 10-week-old ddY mice were used. The animal model of AD was induced by intracerebroventricular (ICV) injection of amyloid ß (Aß) (1–42). CH-3 was orally administrated at a concentration of 250 mg/kg every day starting 2 days before ICV injection. Three weeks after ICV injection, cognitive function was evaluated by Morris Water Maze Test. Brain samples were obtained after behavioral test, and subjected to real-time RT-PCR to measure inflammatory cytokine and NADPH oxidase subunit expression levels. Results: In mice, Aß (1–42) injected by ICV impaired cognitive function compared with vehicle-injected mice. Daily administration of CH-3 significantly attenuated Aß-induced impairment of cognitive function. Aß (1–42) injection enhanced mRNA expressions of inflammatory cytokines such as monocyte chemoattractant protein-1, tumor necrosis factor-alpha, interleukin-6, NADPH oxidase subunits such as p22phox and p47phox, and inducible nitric oxide synthase in hippocampus of mouse brain; whereas treatment with CH-3 tended to reduce these increases of mRNA expressions. Conclusions: Our study demonstrated that cognitive impairment could be improved by repeated oral administration of CH-3. And this ameliorated effect of CH-3 was probably due to prevention of oxidative stress and inflammatory cytokine levels.