Separation of large DNA molecules by applying pulsed electric field to size exclusion chromatography-based microchip. (10th January 2018)

• Record Type: Journal Article
• Authors: Azuma, Naoki; Itoh, Shintaro; Fukuzawa, Kenji; Zhang, Hedong
• Is Part Of: Japanese journal of applied physics. Volume 57:Number 2(2018)
• Publication Date: 2018-01-10
• Subjects: Physics -- Periodicals; 621.05
• DOI: 10.7567/JJAP.57.027002
• Languages: English
• ISSNs: 0021-4922

Abstract

Abstract: Through electrophoresis driven by a pulsed electric field, we succeeded in separating large DNA molecules with an electrophoretic microchip based on size exclusion chromatography (SEC), which was proposed in our previous study. The conditions of the pulsed electric field required to achieve the separation were determined by numerical analyses using our originally proposed separation model. From the numerical results, we succeeded in separating large DNA molecules (λ DNA and T4 DNA) within 1600 s, which was approximately half of that achieved under a direct electric field in our previous study. Our SEC-based electrophoresis microchip will be one of the effective tools to meet the growing demand of faster and more convenient separation of large DNA molecules, especially in the field of epidemiological research of infectious diseases.