Conjugation of epigallocatechin gallate and piperine into a zein nanocarrier: implication on antioxidant and anticancer potential. (14th August 2018)

• Record Type: Journal Article
• Authors: Dahiya, Shakti; Rani, Ruma; Dhingra, Dinesh; Kumar, Sandeep; Dilbaghi, Neeraj
• Is Part Of: Advances in natural sciences. Volume 9:Number 3(2018)
• Publication Date: 2018-08-14
• Subjects: epigallocatechin gallate -- piperine -- zein -- nanoencapsulation -- antioxidant -- haemolytic activity; 2.00 -- 2.04 -- 4.02 -- 5.09; Nanotechnology -- Periodicals; Nanoscience -- Periodicals; 620.5
• DOI: 10.1088/2043-6254/aad5c1
• Languages: English
• ISSNs: 2043-6254

Abstract

Abstract: Epigallocatechin gallate (EGCG), a natural antioxidant present in green tea is a remedy for the treatment of numerous diseases linked to oxidative stress. The aim of the present study was to prepare nanoformulation of EGCG alone and along with piperine into a protein nanocarrier and to study their effect on in vitro antioxidant, haemolytic and anticancer activities. Anti-solvent precipitation method and full-factorial design expert software was used for the synthesis and optimization of EGCG loaded nanosuspension (EGCG NS) and EGCG-piperine nanocomplex (EGCG-Pip NC). The optimized nanosuspensions showed spherical morphology and stable zeta potential. Although an increase in antioxidant potential and a decrease in the haemolytic activity of EGCG were observed due to nanoencapsulation, the EGCG nanoformulation(s) displayed higher anti-cancer activity against various cell lines (HL60, SCC40, MCF7, HeLa and Colo205) in comparison to pure EGCG.