Two-photon lithography and microscopy of 3D hydrogel scaffolds for neuronal cell growth. (16th February 2018)

• Record Type: Journal Article
• Authors: Accardo, Angelo; Blatché, Marie-Charline; Courson, Rémi; Loubinoux, Isabelle; Vieu, Christophe; Malaquin, Laurent
• Is Part Of: Biomedical physics & engineering express. Volume 4:Number 2(2018)
• Publication Date: 2018-02-16
• Subjects: direct laser writing -- cell scaffold -- 3D fabrication -- PEGDA hydrogel -- neuro2A cell -- multi-photon confocal imaging; Medical physics -- Periodicals; Biophysics -- Periodicals; Biomedical engineering -- Periodicals; Medical sciences -- Periodicals; 610.153
• DOI: 10.1088/2057-1976/aaab93
• Languages: English
• ISSNs: 2057-1976

Abstract

Abstract: 3D fabrication techniques are rapidly expanding in the field of scaffold development for cell culture and tissue engineering. Herein we report the realization of free-standing PEGDA hydrogel architectures by using two-photon lithography. The morphological and immunofluorescence characterization of neuro2A cells revealed a tridimensional colonization featuring multiple neuritic extensions per cell as well as the expression of β -tubulin neuronal marker and actin microfilaments. The results open new perspectives in the continuous quest for structured biomaterials able to provide a favorable environment to cells and at the same time not interfering with imaging protocols necessary for a clear scenario of the cell seeding.