Magnetically-focusing biochip structures for high-speed active biosensing with improved selectivity. (2nd May 2018)

• Record Type: Journal Article
• Authors: Yoo, Haneul; Lee, Dong Jun; Kim, Daesan; Park, Juhun; Chen, Xing; Hong, Seunghun
• Is Part Of: Nanotechnology. Volume 29:Number 26(2018)
• Publication Date: 2018-05-02
• Subjects: magnetic trap-and-release -- magnetic nanobead -- high speed immunoassay -- biosensor -- magnetically-focusing; Nanotechnology -- Periodicals; Nanotechnology -- Periodicals; Nanotechnology; Publications périodiques; Nanotechnologies; Periodicals; 620.5
• DOI: 10.1088/1361-6528/aabc4d
• Languages: English
• ISSNs: 0957-4484

Abstract

Abstract: We report a magnetically-focusing biochip structure enabling a single layered magnetic trap-and-release cycle for biosensors with an improved detection speed and selectivity. Here, magnetic beads functionalized with specific receptor molecules were utilized to trap target molecules in a solution and transport actively to and away from the sensor surfaces to enhance the detection speed and reduce the non-specific bindings, respectively. Using our method, we demonstrated the high speed detection of IL-13 antigens with the improved detection speed by more than an order of magnitude. Furthermore, the release step in our method was found to reduce the non-specific bindings and improve the selectivity and sensitivity of biosensors. This method is a simple but powerful strategy and should open up various applications such as ultra-fast biosensors for point-of-care services.