Electrochemical aptasensor for detecting tetracycline in milk. (15th February 2016)

• Record Type: Journal Article
• Authors: Le, Thi Hanh; Pham, Van Phuc; La, Thi Huyen; Phan, Thi Binh; Le, Quang Huan
• Is Part Of: Advances in natural sciences. Volume 7:Number 1(2016)
• Publication Date: 2016-02-15
• Subjects: aptamer -- detection -- EIS -- electrochemical -- tetracycline; 6.09; Nanotechnology -- Periodicals; Nanoscience -- Periodicals; 620.5
• DOI: 10.1088/2043-6262/7/1/015008
• Languages: English
• ISSNs: 2043-6254

Abstract

Abstract: A rapid, simple and sensitive biosensor system for tetracycline detection is very important in food safety. In this paper we developed a label-free aptasensor for electrochemical detection of tetracycline. According to the electrochemical impendence spectroscopy (EIS) analysis, there was a linear relationship between the concentration of tetracycline and the electron transfer resistance from 10 to 3000 ng ml −1 of the tetracycline concentration. The detection limit was 10 ng ml −1 in 15 min detection duration. The prepared aptasensor showed a good reproducibility with an acceptable stability in tetracycline detection. The recoveries of tetracycline in spiked milk samples were in the range of 88.1%–94.2%. The aptasensor has sensitivity 98% and specificity of 100%.