On chip purification of hiPSC-derived cardiomyocytes using a fishnet-like microstructure. (7th September 2016)

• Record Type: Journal Article
• Authors: Li, Xin; Yu, Leqian; Li, Junjun; Minami, Itsunari; Nakajima, Minako; Noda, Yuichiro; Kotera, Hidetoshi; Liu, Li; Chen, Yong
• Is Part Of: Biofabrication. Volume 8:Number 3(2016)
• Publication Date: 2016-09-07
• Subjects: microfluidic -- cell sorting -- cardiomyocytes -- purification -- pluripotent stem cell; Biomedical engineering -- Periodicals; Tissue engineering -- Periodicals; Biomedical materials -- Microstructure -- Periodicals; Bioengineering -- Periodicals; 610.28
• DOI: 10.1088/1758-5090/8/3/035017
• Languages: English
• ISSNs: 1758-5082

Abstract

Abstract: Human induced pluripotent stem cells (hiPSCs) can be differentiated at high efficiency into cells of a targeting type but the resulting cell population has to be of high purity for clinical therapies to avoid teratomas. Herein, we report a microfluidic device with integrated and surface functionalised fishnet-like structures for specific cell capture. With the help of a flow derivation surface pattern, cells in solution are forced to cross the fishnet-like structure, resulting in high efficiency and selective retention of a chosen cell population. A suspension of hiPSCs and hiPSC-derived cardiomyocytes were used for device function validation. We found that a hiPSC capture rate over 80% can be achieved along with a remarkable increase in the CM population rate in the recovered suspension without affecting cell viability.