Multi-platform nano-immunosensor for aflatoxin M1 in milk. (14th April 2015)

• Record Type: Journal Article
• Authors: Pal, Souvik; Sharma, Manoj Kumar; Chatterjee, Ratnamala; Bhand, Sunil
• Is Part Of: Materials research express. Volume 2:Number 4(2015)
• Publication Date: 2015-04-14
• Subjects: hafnia nanoparticles -- self-assembled monolayer -- immunosensor -- chemiluminescence -- photometry -- aflatoxin M1 -- milk; Materials science -- Research -- Periodicals; Materials science -- Periodicals; 620.11
• DOI: 10.1088/2053-1591/2/4/045010
• Languages: English
• ISSNs: 2053-1591

Abstract

Abstract: A multi-platform detection based on hafnia nanoparticles (HfO2 NPs) has been developed for immunosensing of aflatoxin M1 (AFM1) as a model analyte. The fine-grained nanocrystal ceramic powder samples of HfO2 NPs were prepared using a solution based chemical method by easily available laboratory reagents. Highly specific monoclonal antibody was immobilized on HfO2 surface using novel chemical modification and crosslinking chemistry. The nano-immunosensor was investigated using the chemiluminescent sandwich enzyme-linked immunosorbent assay in the range 200–0.5 pg mL −1 . Simultaneously, the particles were also investigated photometrically. The developed immuno-sensor exhibited good linearity with a limit of detection 6.25 pg mL −1, sensitivity, selectivity and stability. The IC50 value was obtained at 61.48 pg mL −1 . Moreover, recovery of milk samples was close to 100%. Using the developed immuno-sensor, AFM1 was measurable even after 30 days at storage condition of 4 °C and as well as room temperature (at 28 °C). The miniaturised nano-immunosensor was used for high-throughput analysis (384 individual samples/40 min) of AFM1. To the best of our knowledge, this is the first report in which HfO2 NPs are used for the fabrication of a multi-platform immuno-sensor system for AFM1 in milk.