Direct plasma irradiation affects expression of RNAs in cultured mammalian cells. (1st November 2016)

• Record Type: Journal Article
• Authors: Kobayashi, Mime; Tokaji, Hideto; Kumagai, Shinya
• Is Part Of: Applied physics express. Volume 9:Number 12(2016:Dec.)
• Publication Date: 2016-11-01
• Subjects: Physics -- Periodicals; Technology -- Periodicals; 621.05
• DOI: 10.7567/APEX.9.127001
• Languages: English
• ISSNs: 1882-0778

Abstract

Abstract: The expression of RNAs in mouse NIH3T3 cells was altered by low-temperature atmospheric-pressure plasma irradiation. Cell culture liquid media were removed before plasma irradiation so that direct plasma effects can be assessed. After 5 s irradiation, the cells were cultured in media for 1 or 3 h and RNA expression was analyzed using a microarray. When analyzed 1 and 3 h after plasma irradiation, the upregulation of hypothetical transmembrane proteins and U3 small nucleolar RNAs was detected at both time points. Our results provide a basic principle for understanding the molecular mechanisms of plasma effects on mammalian cells.