Preservation over time of dried acellular esophageal matrix. (22nd October 2018)

• Record Type: Journal Article
• Authors: Giobbe, Giovanni G; Zambon, Alessandro; Vetralla, Massimo; Urbani, Luca; Deguchi, Koichi; Pantano, Maria F; Pugno, Nicola M; Elvassore, Nicola; De Coppi, Paolo; Spilimbergo, Sara
• Is Part Of: Biomedical physics & engineering express. Volume 4:Number 6(2018)
• Publication Date: 2018-10-22
• Subjects: dry decellularized scaffold -- esophageal replacement -- supercritical CO2 drying -- extracellular matrix preservation -- tissue engineering -- mesenchymal stem cells -- tissue storage; Medical physics -- Periodicals; Biophysics -- Periodicals; Biomedical engineering -- Periodicals; Medical sciences -- Periodicals; 610.153
• DOI: 10.1088/2057-1976/aae4ff
• Languages: English
• ISSNs: 2057-1976

Abstract

Abstract: Preservation of acellular matrices represents a big challenge for the improvement of tissue engineering. In this work, a new method to preserve over time a decellularized esophageal scaffolds was explored. Dried and sterile acellular esophagi were obtained with a combined treatment of ethanol and a subsequent supercritical CO2 drying. Preservation of the extracellular matrix architecture, collagen content, and mechanical properties up to 6 months demonstrated the efficiency of the methodology with implications in natural scaffold storage. In vitro support of mesenchymal stem cells showed a promising indication to the further use of the technology in pre-clinical and clinical application.