Effect of microRNA-29b on proliferation, migration, and invasion of endometrial cancer cells. Issue 8 (August 2019)

• Record Type: Journal Article
• Authors: Kong, Jian; He, Xiuting; Wang, Yan; Li, Jie
• Is Part Of: Journal of international medical research. Volume 47:Issue 8(2019)
• Page Start: 3803
• Page End: 3817
• Publication Date: 2019-08
• Subjects: MicroRNA-29b -- phosphatase and tensin homolog (PTEN) -- AKT -- endometrial cancer -- cisplatin -- chemoresistance; Medicine -- Periodicals; Pharmacology -- Periodicals; 610.5
• DOI: 10.1177/0300060519844403
• Languages: English
• ISSNs: 0300-0605

Abstract

Objective: Aberrant expression of microRNAs is a key regulator of tumorigenesis and progression in endometrial cancer. We assessed the effect of microRNA-29b (miR-29b) on proliferation, chemosensitivity, migration, and invasion of endometrial cancer cells. Methods: The proliferation of endometrial cancer cells was examined by water-soluble tetrazolium (WST)-1 assay. The effects of miR-29b on migration and invasion were evaluated by transwell migration and Matrigel invasion assays. Western blotting was used to assess protein expression levels after altered expression of miR-29b. The effect of miR-29b on cisplatin-induced apoptosis was examined by Caspase-Glo 3/7 assay. Results: miR-29b inhibited proliferation and decreased migration and invasion of endometrial cancer cells. It also enhanced the sensitivity of endometrial cancer cells to cisplatin and increased cisplatin-induced apoptosis by regulating expression of BAX and Bcl-2. Moreover, miR-29b changed the expression level of phosphatase and tensin homolog (PTEN) and p-AKT by directly binding to the 3′ untranslated region of PTEN. Conclusion: miR-29b played important roles in proliferation and progression in endometrial cancer cells by direct regulation of PTEN. It might be used as a biomarker to predict chemotherapy response and prognosis in endometrial cancer.