A standard addition method to quantify histamine by reductive amination and hydrophilic interaction liquid chromatography coupled with tandem mass spectrometry. Issue 5 (October 2019)

• Record Type: Journal Article
• Authors: Shen, Po-Tsun; Lin, Yi-Reng; Chen, Bing-Hung; Huang, Mei-Fang; Cheng, Chieh-Wen; Shiue, Yow-Ling; Liang, Shih-Shin
• Is Part Of: European journal of mass spectrometry. Volume 25:Issue 5(2019)
• Page Start: 412
• Page End: 418
• Publication Date: 2019-10
• Subjects: Standard addition method -- histamine -- reductive amination -- multiple reaction monitoring -- hydrophilic interaction liquid chromatography -- mass spectrometry; Mass spectrometry -- Periodicals; Mass Spectrometry; Mass spectrometry; Periodicals; Periodicals; 543.6505
• DOI: 10.1177/1469066719838966
• Languages: English
• ISSNs: 1469-0667

Abstract

Histamine is an organic nitrogenous compound that acts as a neurotransmitter in the uterus, spinal cord, and brain and is involved in local immune responses. In this study, we developed a fast and simple derivatization method based on reductive amination that can be used to quantify histamine by hydrophilic interaction liquid chromatography coupled with tandem mass spectrometry. Histamine isotope analogs were synthesized via reductive amination. Histamine was modified with H2 -formaldehyde to form N -dimethylated histamine to act as a standard or with D2 -formaldehyde to form N -dimethylated histamine- d 4 to act as an internal standard. Using this method, we achieved a limit of detection of 3.6 ng/mL, a limit of quantification of 7.9 ng/mL, and a linear calibration curve with a coefficient of determination (R 2 ) of 0.9987. Furthermore, the intra-day relative standard deviations ranged from 0.9% to 3.7% and the inter-day relative standard deviations ranged from 2.0% to 17.6%. After derivatization, N -dimethylated histamine showed 382.5% signal enhancement compared to unmodified histamine in mass spectrometry detection. To demonstrate the applicability of this method for biological samples, we utilized standard addition method to quantify histamine in fetal bovine serum and achieved a recovery of 86.7%.