Spatial organization of the somatosensory cortex revealed by osmFISH. (November 2018)

• Record Type: Journal Article
• Authors: Codeluppi, Simone; Borm, Lars; Zeisel, Amit; Manno, Gioele; Lunteren, Josina; Svensson, Camilla; Linnarsson, Sten
• Is Part Of: Nature methods. Volume 15:Number 11(2018)
• Page Start: 932
• Page End: 935
• Publication Date: 2018-11
• Subjects: Life sciences -- Methodology -- Periodicals; Life sciences -- Research -- Periodicals; Biology -- Methodology -- Periodicals; Biology -- Research -- Periodicals; 570.72
• DOI: 10.1038/s41592-018-0175-z
• Languages: English
• ISSNs: 1548-7091

Abstract

Abstract Global efforts to create a molecular census of the brain using single-cell transcriptomics are producing a large catalog of molecularly defined cell types. However, spatial information is lacking and new methods are needed to map a large number of cell type–specific markers simultaneously on large tissue areas. Here, we describe a cyclic single-molecule fluorescence in situ hybridization methodology and define the cellular organization of the somatosensory cortex. osmFISH applies automated cycles of single-molecule fluorescence in situ hybridization without barcoding to provide spatial gene expression in tissue sections at high sensitivity, accuracy and throughput.