Opposing effects of final population density and stress on Escherichia coli mutation rate. Issue 12 (December 2018)

• Record Type: Journal Article
• Authors: Krašovec, Rok; Richards, Huw; Gifford, Danna; Belavkin, Roman; Channon, Alastair; Aston, Elizabeth; McBain, Andrew; Knight, Christopher
• Is Part Of: ISME journal. Volume 12:Issue 12(2018)
• Page Start: 2981
• Page End: 2987
• Publication Date: 2018-12
• Subjects: Microbial ecology -- Periodicals; 579.1705
• DOI: 10.1038/s41396-018-0237-3
• Languages: English
• ISSNs: 1751-7362

Abstract

Abstract Evolution depends on mutations. For an individual genotype, the rate at which mutations arise is known to increase with various stressors (stress-induced mutagenesis—SIM) and decrease at high final population density (density-associated mutation-rate plasticity—DAMP). We hypothesised that these two forms of mutation-rate plasticity would have opposing effects across a nutrient gradient. Here we test this hypothesis, culturingEscherichia coli in increasingly rich media. We distinguish an increase in mutation rate with added nutrients through SIM (dependent on error-prone polymerases Pol IV and Pol V) and an opposing effect of DAMP (dependent on MutT, which removes oxidised G nucleotides). The combination of DAMP and SIM results in a mutation rate minimum at intermediate nutrient levels (which can support 7 × 108 cells ml−1 ). These findings demonstrate a strikingly close and nuanced relationship of ecological factors—stress and population density—with mutation, the fuel of all evolution.