Cross-feeding modulates antibiotic tolerance in bacterial communities. Issue 11 (November 2018)

• Record Type: Journal Article
• Authors: Adamowicz, Elizabeth; Flynn, Jeffrey; Hunter, Ryan; Harcombe, William
• Is Part Of: ISME journal. Volume 12:Issue 11(2018)
• Page Start: 2723
• Page End: 2735
• Publication Date: 2018-11
• Subjects: Microbial ecology -- Periodicals; 579.1705
• DOI: 10.1038/s41396-018-0212-z
• Languages: English
• ISSNs: 1751-7362

Abstract

Abstract Microbes frequently rely on metabolites excreted by other bacterial species, but little is known about how this cross-feeding influences the effect of antibiotics. We hypothesized that when species rely on each other for essential metabolites, the minimum inhibitory concentration (MIC) for all species will drop to that of the "weakest link"—the species least resistant in monoculture. We tested this hypothesis in an obligate cross-feeding system that was engineered betweenEscherichia coli, Salmonella enterica, andMethylobacterium extorquens . The effect of tetracycline and ampicillin were tested on both liquid and solid media. In all cases, resistant species were inhibited at significantly lower antibiotic concentrations in the cross-feeding community than in monoculture or a competitive community. However, deviation from the "weakest link" hypothesis was also observed in cross-feeding communities apparently as result of changes in the timing of growth and cross-protection. Comparable results were also observed in a clinically relevant system involving facultative cross-feeding betweenPseudomonas aeruginosa and an anaerobic consortium found in the lungs of cystic fibrosis patients.P. aeruginosa was inhibited by lower concentrations of ampicillin when cross-feeding than when grown in isolation. These results suggest that cross-feeding significantly alters tolerance to antibiotics in a variety of systems.