Degradation of Asphaltenic Fraction by Locally Isolated Halotolerant Bacterial Strains. (9th April 2012)

• Record Type: Journal Article
• Authors: Ali, Hager R.; El-Gendy, Nour Sh.; Moustafa, Yasser M.; Roushdy, Mohamed I.; Hashem, Ahmed I.
• Other Names: Allred B. J. Academic Editor.; Cutright T. J. Academic Editor.; Ding W. Academic Editor.; He Z. L. Academic Editor.
• Is Part Of: ISRN soil science. Volume 2012(2012)
• Publication Date: 2012-04-09
• Subjects: Soil science -- Periodicals; Soils -- Research -- Periodicals; Soil science; Soils -- Research; Soil Sciences; Periodicals; Electronic journals; 631.4
• DOI: 10.5402/2012/435485
• Languages: English
• ISSNs: 2090-875X

Abstract

Abstract : Three halotolerant bacterial species were isolated from locally oil-polluted water sample for their ability to utilize asphaltene (Asph) fraction as sole carbon and energy source. These bacteria degrade 83–96% of 2500 mg/L asphaltene within 21 d at30 ° C and pH7. They were identified as Bacillus sp. Asph1, Pseudomonas aeruginosa Asph2, and Micrococcus sp. Asph3. A statistically significant difference at 95% confidence level for cell growth and percentage biodegradation (%BD) was observed in all biotreatment flasks relative to noninoculated (–ve control) flasks. Regression analysis estimated a quadratic polynomial equation for Asph biodegradation as a function of cell growth. Gel permeation chromatographic (GPC) and Fourier transform infrared (FT-IR) analysis revealed decrease in Asph average molecular weights and significant alternations in functional groups after biotreatment, respectively.