Supplementation of maturation medium with CoQ10 enhances developmental competence of ovine oocytes through improvement of mitochondrial function. Issue 7 (7th May 2019)

• Record Type: Journal Article
• Authors: Heydarnejad, Alireza; Ostadhosseini, Somayyeh; Varnosfaderani, Shiva Rouhollahi; Jafarpour, Farnoosh; Moghimi, Ali; Nasr‐Esfahani, Mohammad Hossein
• Is Part Of: Molecular reproduction and development. Volume 86:Issue 7(2019)
• Page Start: 812
• Page End: 824
• Publication Date: 2019-05-07
• Subjects: coenzyme Q10 -- in vitro maturation -- mitochondrial status -- oocytes -- ovine -- oxidative stress; Reproduction -- Periodicals; Molecular biology -- Periodicals; Molecular genetics -- Periodicals; Embryology -- Periodicals; 571.8
• DOI: 10.1002/mrd.23159
• Languages: English
• ISSNs: 1040-452X

Abstract

Abstract: In vitro maturation (IVM) can impair the balance between antioxidant capacity and oxidative stress, and jeopardize embryo development by increasing oxidative stress, reducing energy metabolism, and causing improper meiotic segregation. Balancing the energy production and reduction of oxidative stress can be achieved by supplementation with coenzyme Q10 (CoQ10), an electron transporter in the mitochondrial inner membrane. To improve the in vitro production of ovine embryos, we studied the effect of CoQ10 supplementation during the maturation of sheep oocytes. A minimum of 100 cumulus‐oocyte complexes (COCs) were matured in the presence of 15, 30, or 50 μM CoQ10 in three to five replicates; next, in vitro fertilization and culture in a subset of oocytes were done. Our data revealed that compared to control oocytes or other concentrations of CoQ10, supplementation with 30 µM CoQ10 resulted in a significant increase in blastocyst formation and hatching rates, improved the distribution, relative mass and potential membrane of mitochondria, decreased the levels of reactive oxygen species and glutathione and lessened the percentage of oocytes with misaligned chromosomes after spindle assembly. The relative expression levels of apoptosis markers CASPASE3 and BAX were significantly reduced in CoQ10‐treated oocytes and cumulus cells whereas the relative expression level of GDF9, an oocyte‐specific growth factor, significantly increased. In conclusion, supplementation with CoQ10 improves the quality of COCs and the subsequent developmental competence of the embryo. Abstract : In this study, we have shown that exposure of oocytes to 30 µM coenzyme Q10 (CoQ10), as an antioxidant and bioenergizer, during oocyte maturation increased the level of blastocyst and hatching rates. Addition of 30 µM CoQ10 improved distribution, relative mass, and potential membrane of mitochondria against control group. Furthermore, our results demonstrated that intracellular reactive oxygen species and glutathione levels decreased in 30 μM CoQ10 group against control. In addition, the percentage of misaligned chromosomes was decreased following treatment with CoQ10.