Digital morphology analyzers in hematology: ICSH review and recommendations. (2nd May 2019)

• Record Type: Journal Article
• Authors: Kratz, Alexander; Lee, Szu‐hee; Zini, Gina; Riedl, Jurgen A.; Hur, Mina; Machin, Sam
• Is Part Of: International journal of laboratory hematology. Volume 41:Number 4(2019:Aug.)
• Page Start: 437
• Page End: 447
• Publication Date: 2019-05-02
• Subjects: digital imaging -- laboratory hematology -- laboratory standards -- recommendations; Hematology -- Periodicals; Blood -- Diseases -- Periodicals; Hematology -- Periodicals; 616.15005
• DOI: 10.1111/ijlh.13042
• Languages: English
• ISSNs: 1751-5521

Abstract

Abstract: Introduction: Morphological assessment of the blood smear has been performed by conventional manual microscopy for many decades. Recently, rapid progress in digital imaging and information technology has led to the development of automated methods of digital morphological analysis of blood smears. Methods: A panel of experts in laboratory hematology reviewed the literature on the use of digital imaging and other strategies for the morphological analysis of blood smears. The strengths and weaknesses of digital imaging were determined, and recommendations on improvement were proposed. Results: By preclassifying cells using artificial intelligence algorithms, digital image analysis automates the blood smear review process and enables faster slide reviews. Digital image analyzers also allow remote networked laboratories to transfer images rapidly to a central laboratory for review, and facilitate a variety of essential work functions in laboratory hematology such as consultations, digital image archival, libraries, quality assurance, competency assessment, education, and training. Different instruments from several manufacturers are available, but there is a lack of standardization of staining methods, optical magnifications, color and display characteristics, hardware, software, and file formats. Conclusion: In order to realize the full potential of Digital Morphology Hematology Analyzers, pre‐analytic, analytic, and postanalytic parameters should be standardized. Manufacturers of new instruments should focus on improving the accuracy of cell preclassifications, and the automated recognition and classification of pathological cell types. Cutoffs for grading morphological abnormalities should depend on clinical significance. With all current devices, a skilled morphologist remains essential for cell reclassification and diagnostic interpretation of the blood smear.