Optical control of cell differentiation on synthetic collagen-like scaffolds. Issue 13 (2nd September 2019)

• Record Type: Journal Article
• Authors: Kobayashi, Mime; Honda, Ryosuke; Ando, Tsuyoshi; Tanihara, Masao
• Is Part Of: Journal of biomaterials science. Volume 30:Issue 13(2019)
• Page Start: 1161
• Page End: 1171
• Publication Date: 2019-09-02
• Subjects: Biocompatible -- scaffolds -- stem cells -- biomaterials -- tissue engineering -- photoinitiators; Polymers -- Biocompatibility -- Periodicals; Biomedical materials -- Periodicals; 572.33
• DOI: 10.1080/09205063.2019.1622845
• Languages: English
• ISSNs: 0920-5063

Abstract

Abstract: We have developed biocompatible scaffolds that enable cell fate control with visible light. The scaffolds are based on synthetic collagen-like polypeptide, poly(prolyl-hydroxyprolyl-glycyl) {poly(Pro-Hyp-Gly)} which has been used for cosmetics and other healthcare applications. Bioactive peptides were conjugated to the scaffolds via photoactivation reaction utilizing 488 nm visible light. In addition, the use of a photocleavable crosslinker enables dissociation of chemical moieties by 405 nm laser irradiation. The synthesis scheme enables optical control to attach and detach functional peptides in pre-patterned shapes. Using bone forming peptide (BFP), we demonstrate that calcium deposition by rat bone stromal cells can be directed on the scaffold. Using other signaling molecules and three-dimensional scaffolds, controlled differentiation of stem cells can be achieved by spatio-temporally specific irradiation of confocal microscope laser.