Granulatosides D, E and other polar steroid compounds from the starfish Choriaster granulatus. Their immunomodulatory activity and cytotoxicity. Issue 18 (17th September 2019)

• Record Type: Journal Article
• Authors: Ivanchina, Natalia V.; Kicha, Alla A.; Malyarenko, Timofey V.; Ermolaeva, Sofiya D.; Yurchenko, Ekaterina A.; Pislyagin, Evgeny A.; Van Minh, Chau; Dmitrenok, Pavel S.
• Is Part Of: Natural product research. Volume 33:Issue 18(2019)
• Page Start: 2623
• Page End: 2630
• Publication Date: 2019-09-17
• Subjects: Polyhydroxysteroids -- glycosides -- starfish -- Choriaster granulatus -- cytotoxicity -- immunomodulatory activity; Natural products -- Periodicals; Natural products -- Research -- Periodicals; Biological Factors -- Periodicals; Biological Products -- Periodicals; 547
• DOI: 10.1080/14786419.2018.1463223
• Languages: English
• ISSNs: 1478-6419

Abstract

Abstract: Two new steroid glycosides, granulatosides D (1 ) and E (2 ), belonging to the group of bi- and monoglycosides of polyhydroxysteroids, respectively, were isolated from the ethanolic extract of the starfish Choriaster granulatus along with thirteen previously known glycosides of polyhydroxysteroids (3 –15 ) and one steroid heptaol (16 ). The structures of the new compounds were elucidated by extensive NMR and ESIMS techniques. Cytotoxic and immunomodulatory activities of compounds1, 3 –8, and10 –16 using murine splenocytes and peritoneal macrophages were studied. At a dose of 0.1 μM new glycoside1 showed immunomodulatory properties, increasing the intracellular ROS (reactive oxygen species) level in peritoneal murine macrophages by 20% and decreasing intracellular ROS level by 21% in pre-treated with endotoxic lipopolysaccharide from E. coli (LPS) peritoneal macrophages. Abstract :