Branch migration based selective PCR for DNA mutation enrichment and detection. Issue 58 (2nd July 2019)

• Record Type: Journal Article
• Authors: Chen, Na; Ouyang, Xiaofei; Lin, Meng; Liu, Na; Wu, Tongbo; Xiao, Xianjin
• Is Part Of: Chemical communications. Volume 55:Issue 58(2019)
• Page Start: 8466
• Page End: 8469
• Publication Date: 2019-07-02
• Subjects: Chemistry -- Periodicals; 540
• DOI: 10.1039/c9cc04161c
• Languages: English
• ISSNs: 1359-7345

Abstract

Abstract : A selective PCR for the enrichment of low-abundance mutations by introducing a branch migration blocker into the PCR process. Abstract : We presented a branch migration based PCR in which a branch migration blocker was introduced to selectively reduce the amplification efficiency of the wild-type target and enrich the mutant-type target. The low-abundance mutations could be enriched and then detected by high resolution melting, Sanger sequencing or fluorescent DNA probe.