A mitochondria-targeted two-photon fluorogenic probe for the dual-imaging of viscosity and H2O2 levels in Parkinson's disease models. Issue 27 (24th June 2019)

• Record Type: Journal Article
• Authors: Li, Hao; Xin, Chenqi; Zhang, Gaobin; Han, Xisi; Qin, Wenjing; Zhang, Cheng-wu; Yu, Changmin; Jing, Su; Li, Lin; Huang, Wei
• Is Part Of: Journal of materials chemistry. Volume 7:Issue 27(2019)
• Page Start: 4243
• Page End: 4251
• Publication Date: 2019-06-24
• Subjects: Materials -- Periodicals; Chemistry, Analytic -- Periodicals; Biomedical materials -- Research -- Periodicals; 543.0284
• DOI: 10.1039/c9tb00576e
• Languages: English
• ISSNs: 2050-750X

Abstract

Abstract : Novel two-photon fluorogenic probe could simultaneously monitor changes in the mitochondrial viscosity and H2 O2 levels using two different channels. Abstract : Parkinson's disease (PD) is a neurodegenerative disease that seriously affects the quality of life of the patient. Many sources have shown that mitochondrial dysfunction is increasingly appreciated as playing a critical role in the pathogenesis of PD. The mitochondrial microenvironment and H2 O2 level are two key parameters that are used to assess the function of mitochondria. Herein, we designed and synthesized a novel two-photon (TP) fluorogenic probeMito-LX, which could simultaneously monitor changes in the mitochondrial viscosity and H2 O2 levels using two different channels. This probe showed a significant response to viscosity changes with red emission at 730 nm and high sensitivity to H2 O2 levels with orange emission at 585 nm. Moreover, Mito-LX was successfully applied to living systems (including living cells, zebrafish and Drosophila ) for viscosity and H2 O2 detection using one- and two-photon fluorescence confocal microscopy.