Experimental composites containing quaternary ammonium methacrylates reduce demineralization at enamel-restoration margins after cariogenic challenge. Issue 8 (August 2019)

• Record Type: Journal Article
• Authors: Maia, André Coimbra; Mangabeira, Adrielle; Vieira, Renato; Neves, Aline de Almeida; Lopes, Ricardo Tadeu; Pires, Thais Maria; Viana, Gil Mendes; Cabral, Lúcio Mendes; Cavalcante, Larissa Maria; Portela, Maristela Barbosa
• Is Part Of: Dental materials. Volume 35:Issue 8(2019)
• Page Start: e175
• Page End: e183
• Publication Date: 2019-08
• Subjects: Dental composite -- Quaternary ammonium -- Restorative dentistry -- Streptococcus mutans -- Demineralized enamel -- Biofilm; Dentistry -- Periodicals; Dental materials -- Periodicals; 617.695
• DOI: 10.1016/j.dental.2019.05.021
• Languages: English
• ISSNs: 0109-5641

Abstract

Abstract: Objective: This study evaluated the influence of experimental composites containing quaternary ammonium monomers (QAM) at different concentrations and alkyl chains on demineralization at enamel-composite margins after cariogenic challenge. Methods: Standardized 4 × 4 mm cavities were cut into 35 bovine enamel blocks, which were randomly divided into seven groups (n = 5) and restored with the following experimental composites and commercial materials: (G12.5) – 5% dimethylaminododecyl methacrylate (DMADDM) with a 12-carbon alkyl chain (G12.10) – 10% DMADDM, (G16.5) – 5% dimethylaminohexadecyl methacrylate (DMAHDM) with a 16-carbon alkyl chain (G16.10) – 10% DMAHDM, (CG) – control group (without QAM), (GZ250) – commercial composite (Filtek Z250 ® ), and (GIC) – glass ionomer cement (Maxxion R ® ). After restorative procedures, initial microhardness was measured and experimental composites were subjected to Streptococcus mutans biofilm formation for 48 h. After cariogenic challenge, the samples were washed and microhardness was reassessed. A 3D non-contact profilometer was used to determine surface roughness and enamel demineralization was assessed by micro-CT. Microhardness results were analyzed by the Kruskal–Wallis and Mann-Whitney tests and micro-CT results were analyzed by Tukey's HSD test (95% confidence interval). Results: None of the materials could prevent mineral loss at the enamel-restoration margins. The addition of 10% DMAHDM yielded the lowest, albeit statistically significant, mineral loss (p < 0.05). 3D non-contact profilometry showed enamel surface roughness modification after biofilm exposure. The CG had the highest roughness values. Micro-CT analysis revealed mineral loss, except for GIC. Significance: The addition of 10% QAM with a 16-carbon chain in experimental composites reduced mineral loss at the enamel-restoration margins after cariogenic challenge.