Software-Based Improvement of Image Information Using Thrombocytes as a Practical Demonstration. (July 2019)

• Record Type: Journal Article
• Authors: Piper, J.
• Is Part Of: Microscopy today. Volume 27:Number 4(2019)
• Page Start: 12
• Page End: 16
• Publication Date: 2019-07
• Subjects: dark-field microscopy, -- digital image enhancement, -- S-spline algorithm, -- thrombocytes, -- pseudopodia; Microscope and microscopy -- Periodicals; 502.8
• DOI: 10.1017/S1551929519000634
• Languages: English
• ISSNs: 1551-9295

Abstract

Abstract: Abstract: Fine details of morphological changes in biological structures, which approach the resolution limits of a microscope or detection device, are often difficult to determine. These difficulties are compounded if imaging of living specimens is required. In this work we use a variety of software approaches to enhance individual images removed from a video of thrombocyte (platelet) activation on a glass coverslip. Through software approaches, fine details and focal depth were significantly improved in images of pseudopodia from activated thrombocytes.