Visualization and quantification of cellular RNA production and degradation using a combined fluorescence and mass spectrometry characterization assay. Issue 57 (1st July 2019)

• Record Type: Journal Article
• Authors: Gao, Xiaoying; Shu, Xiao; Song, Yinuo; Cao, Jie; Gao, Minsong; Wang, Fengqin; Wang, Yizhen; Sun, Jing Zhi; Liu, Jianzhao; Tang, Ben Zhong
• Is Part Of: Chemical communications. Volume 55:Issue 57(2019)
• Page Start: 8321
• Page End: 8324
• Publication Date: 2019-07-01
• Subjects: Chemistry -- Periodicals; 540
• DOI: 10.1039/c9cc03923f
• Languages: English
• ISSNs: 1359-7345

Abstract

Abstract : A combined fluorescence and mass spectrometry assay is developed to visualize and quantify cellular RNA production and degradation. Abstract : Here we report a combined fluorescence and mass spectrometry assay which is capable of stably visualizing and quantifying cellular nucleoside-labeled RNA production and degradation. The fluorescence and mass spectrometry signals from cellular labeled RNAs show a linear correlation. This simple and robust assay benefits the biological community to study RNA metabolism.