Development and validation of a capillary electrophoresis method applied to the analysis of l‐citrulline in an oral formulation for pediatric use. Issue 12 (30th April 2019)

• Record Type: Journal Article
• Authors: Salvo, Leandro Javier; Coiana, Lorenzo; Dobrecky, Cecilia; Tripodi, Valeria; Flor, Sabrina; Lucangioli, Silvia
• Is Part Of: Electrophoresis. Volume 40:Issue 12/13(2019)
• Page Start: 1719
• Page End: 1721
• Publication Date: 2019-04-30
• Subjects: Capillary electrophoresis -- l‐citrulline -- MEKC -- Pediatric -- UV‐detection; Electrophoresis -- Periodicals; Electrophoresis -- Periodicals; 541.372
• DOI: 10.1002/elps.201900008
• Languages: English
• ISSNs: 0173-0835

Abstract

Abstract: A simple and highly sensitive CE–UV method was applied in the determination ofl ‐ctrulline, which was developed from an oral formulation for pediatric use. The novel method was based on the analysis ofl ‐citrulline for direct ultraviolet detection at 198 nm. The BGE consisted of 10 mM sodium tetraborate and 50 mM SDS at pH 9, and the electrophoretic parameters were optimized. The method was validated in terms of specificity, linearity, LOD, LOQ, precision, accuracy, and robustness. The LOD and LOQ obtained were 1.36 and 4.54 μg/mL, respectively. In addition, the method offers higher sensitivity and specificity compared with the results obtained from HPLC method using UV‐detectors, in whichl‐ citrulline needs to be derivatizated. Furthermore, low cost and simplicity of the system allowed the rapid and simple quantitation ofl‐ citrulline in the oral formulation for quality control and stability indicated method.