Genome-wide association study of the oil content in upland cotton (Gossypium hirsutum L.) and identification of GhPRXR1, a candidate gene for a stable QTLqOC-Dt5-1. (September 2019)

• Record Type: Journal Article
• Authors: Ma, Jianjiang; Liu, Ji; Pei, Wenfeng; Ma, Qifeng; Wang, Nuohan; Zhang, Xia; Cui, Yupeng; Li, Dan; Liu, Guoyuan; Wu, Man; Zang, XinShan; Song, Jikun; Zhang, Jinfa; Yu, Shuxun; Yu, Jiwen
• Is Part Of: Plant science. Volume 286(2019)
• Page Start: 89
• Page End: 97
• Publication Date: 2019-09
• Subjects: ACCase acetyl-CoA carboxylase -- BILs backcross inbred lines -- CK negative control -- CV coefficient of variation -- DEGs differentially expressed genes -- DF degree of freedom -- DPA days post anthesis -- FAs fatty acids -- GC gas chromatography -- GLM general linear model -- GO gene ontology -- GWAS genome-wide association study -- H2 broad-sense heritability -- IF2 immortalized F2 population -- KEGG Kyoto Encyclopedia of Genes and Genomes -- LPAAT lysophosphatidic acid acyltransferase -- MS mean square -- Max maximum value -- Min minimum value -- OC oil content -- PEPC phosphoenolpyruvate carboxylase -- PRXR peroxidase -- PV phenotypic variation -- QTL quantitative trait locus -- RILs recombinant inbred lines -- SD standard deviation -- SS standard deviation square -- VIGS virus-induced gene silencing -- WRI wrinkled; Gossypium hirsutum -- Oil content -- GWAS -- Transcriptome -- GhPRXR1; Botany -- Periodicals; Botanique -- Périodiques; 580
• DOI: 10.1016/j.plantsci.2019.05.019
• Languages: English
• ISSNs: 0168-9452

Abstract

Highlights: Thirteen cottonseed oil content QTLs associated with 53 common SNPs on 13 chromosomes were identified in multiple environments. Through a combination of transcriptome and qRT-PCR analyses, the novel gene GhPRXR1, located in the stable QTLqOC-Dt5-1, was identified. By VIGS in upland cotton and overexpression in yeast, we found that GhPRXR1 influenced oil accumulation. Abstract: Cottonseed oil is one of the most important renewable resources for edible oil and biodiesel. To detect QTLs associated with cottonseed oil content (OC) and identify candidate genes that regulate oil biosynthesis, a panel of upland cotton germplasm lines was selected among those previously used to perform GWASs in China. In the present study, 13 QTLs associated with 53 common SNPs on 13 chromosomes were identified in multiple environments based on 15, 369 polymorphic SNPs using the Cotton63 KSNP array. Of these, the OC QTL qOC-Dt5-1 delineated by nine SNPs occurred in a confidence interval of 4 SSRs with previously reported OC QTLs. A combined transcriptome and qRT-PCR analysis revealed that a peroxidase gene ( GhPRXR1 ) was predominantly expressed during the middle-late stage (20–35 days post anthesis) of ovule development. The overexpression of GhPRXR1 in yeast significantly increased the OC by 20.01–37.25 %. Suppression of GhPRXR1 gene expression in the virus-induced gene-silenced cotton reduced the OC by 18.11%. Our results contribute to identifying more OC QTLs and verifying a candidate gene that influences cottonseed oil biosynthesis.