An antiviral drug screening system for enterovirus 71 based on an improved plaque assay: A potential high‐throughput method. Issue 8 (1st April 2019)

• Record Type: Journal Article
• Authors: Yin, Yingxian; Xu, Yi; Ou, Zhiying; Yang, Xiangling; Liu, Huanliang
• Is Part Of: Journal of medical virology. Volume 91:Issue 8(2019)
• Page Start: 1440
• Page End: 1447
• Publication Date: 2019-04-01
• Subjects: antiviral drug screening -- enterovirus 71 -- plaque assay -- plaque inhibitory test; Virology -- Periodicals; 616
• DOI: 10.1002/jmv.25463
• Languages: English
• ISSNs: 0146-6615

Abstract

Abstract: Plaque assay plays an irreplaceable role in a variety of virological studies, including determining titers of viruses. Our previous study showed that a simple and highly repeatable plaque assay could be used for enterovirus 71 (EV‐A71). Now, we show that using a subclone of a clinical EV‐A71 isolate and a rhabdomyosarcoma cell line (RD), a plaque assay based on an EV‐A71/RD model could exhibit the most rapid formation of plaques (<2 days), with much higher repeatability and consistency. Inspired by a plaque inhibitory test for testing ribavirin and interferon, as well as a plaque reduction neutralization test, this modified method has been used to establish a convenient system by using 96‐well plates for screening anti‐EV‐A71 drugs from a 130‐compound library containing multiple types of inhibitors. Nine candidate effective compounds for EV‐A71 have been screened out, and among them, nobiletin ( flavonoid ) was found to be a novel effective compound at the concentration of 10 μM. Our findings imply that this improved method based on an EV‐A71/RD model proved to be a potential high‐throughput method in screening novel antiviral drugs for EV‐A71. Undoubtedly, this method can also be applied to other viruses that can produce an obvious cytopathic effect. Highlights: This drug screening system is a convenient tool to screen out effective inhibitors for enterovirus 71(EV‐A71). Nine candidate inhibitors had been screened out from a 130‐compounds library, and six were firstly discovered. Nobiletin was found to be effective to EV‐A71 at low concentration. This drug screening system may be developed to a high‐throughput tool for EV‐A71 and other viruses.