The effect of Epigallocatechin‐3‐gallate on small intestinal morphology, antioxidant capacity and anti‐inflammatory effect in heat‐stressed broilers. Issue 4 (31st January 2019)

• Record Type: Journal Article
• Authors: Song, Jiao; Lei, Xin; Luo, Jingxian; Everaert, Nadia; Zhao, Guiping; Wen, Jie; Yang, Ye
• Is Part Of: Journal of animal physiology and animal nutrition. Volume 103:Issue 4(2019)
• Page Start: 1030
• Page End: 1038
• Publication Date: 2019-01-31
• Subjects: anti‐inflammatory -- antioxidant capacity -- broiler -- Epigallocatechin‐3‐gallate -- heat stress -- intestinal barrier; Animal nutrition -- Periodicals; Feeds -- Periodicals; 636.085
• DOI: 10.1111/jpn.13062
• Languages: English
• ISSNs: 0931-2439

Abstract

Abstract: This study was to investigate the effects of Epigallocatechin‐3‐gallate (EGCG) on intestinal morphology, antioxidant capacity and anti‐inflammatory response in heat‐stressed broiler. A total of 192 2‐week‐old Arbour Acres broilers chickens were divided into four groups with six replicates per group and eight chickens per replicate: one thermoneutral control group (28°C, group TN), which was fed the basal diet; and three cyclic high‐temperature groups (35°C from 7:00 to 19:00 hr; 28°C from 19:00 hr to 7:00 hr, heat stress group), which were fed the basal diet supplementation with EGCG 0 mg/kg (group HS0), 300 mg/kg (group HS300) and 600 mg/kg (group HS600). The gut morphology and intestinal mucosal oxidative stress indicators, as well as intestinal barrier‐related gene expression, were analysed. The results showed that compared with group TN, heat stress reduced the villus height (VH), activities of glutathione peroxidase (GSH‐Px), superoxide dismutase (SOD)and catalase (CAT), increased the crypt depth (CD) and malondialdehyde (MDA)content at 21, 28 and 35 days ( p < 0.05). After the heat‐stressed broilers were supplemented with EGCG, VH, VH/CD (V/C), and the activities of GSH‐Px, SOD and CAT were increased, and CD and MDA content were reduced compared with those in group HS0 without EGCG supplementation at 21, 28 and 35 days ( p < 0.05). The EGCG supplementation promoted the gene expression of nuclear factor‐erythroid 2‐related factor 2 ( Nrf2 ), Claudin‐1, Mucin 2 ( Muc2 ) and alleviated the nuclear factor‐kappa B ( NF‐κB ) and lipopolysaccharide‐induced tumour necrosis factor ( LITAF ) gene expression compared with group HS0 ( p < 0.05). Moreover, intestinal morphology was strongly correlated with antioxidant ability and inflammatory response. In conclusion, EGCG alleviated the gut oxidative injury of heat‐stressed broilers by enhancing antioxidant capacity and inhibiting inflammatory response.