Early physiological and biochemical responses of soyabean to neighbouring weeds under resource‐independent competition. (3rd May 2019)

• Record Type: Journal Article
• Authors: McKenzie‐Gopsill, A G; Amirsadeghi, S; Earl, H J; Jones, A M P; Lukens, L; Lee, E; Swanton, C J
• Editors: Tei, Francesco
• Is Part Of: Weed research. Volume 59:Number 4(2019)
• Page Start: 288
• Page End: 299
• Publication Date: 2019-05-03
• Subjects: biomass production -- carbohydrate partitioning -- far‐red light -- photosynthesis -- reactive oxygen species -- soyabean–weed interaction; Weeds -- Control -- Periodicals; Herbicides -- Periodicals; 632.5
• DOI: 10.1111/wre.12365
• Languages: English
• ISSNs: 0043-1737

Abstract

Summary: The mechanisms by which weeds compete with crop plants are poorly understood. To gain insight into these mechanisms, we characterised early physiological responses of soyabean to neighbouring weeds using a biological weedy system that generated a consistent far‐red‐enriched light environment and excluded direct resource competition. Neighbouring weeds decreased superoxide dismutase activity in unifoliate leaves. This coincided with increased hydrogen peroxide (H2 O2 ) and oxidized ascorbate levels, while the steady‐state level of superoxide, catalase activity and lipid peroxidation remained unchanged. These responses suggested increased leaf production of singlet oxygen ( 1 O2 ), which was demonstrated by detection of increased Singlet Oxygen Sensor Green (SOSG) fluorescence within 3 h after staining of unifoliate leaves. This finding was further supported by increased ratios of the photosensitiser protochlorophyllide to both chlorophyllide a, and total chlorophyll in the dark as well as enhanced sensitivity to cell death by a 1 O2 ‐generating compound in the light. These responses coincided with dramatic changes in photosynthesis, carbon partitioning and biomass allocation with a persistent decline in leaf sucrose level and biomass production at later growth stages. This study provides direct experimental evidence that under resource‐independent competition, far‐red‐enriched light reflected by neighbouring weeds can alter the balance between ROS production and detoxification and thereby generate an oxidative stress signal in soyabean leaves.