Longitudinal study of t‐cell somatic mutations conferring glycosylphosphatidylinositol‐anchor deficiency in gulf war I veterans exposed to depleted uranium. (27th March 2019)

• Record Type: Journal Article
• Authors: Albertini, Richard J.; Nicklas, Janice A.; Vacek, Pamela M.; Carter, Elizabeth W.; McDiarmid, Melissa
• Is Part Of: Environmental and molecular mutagenesis. Volume 60:Number 6(2019)
• Page Start: 494
• Page End: 504
• Publication Date: 2019-03-27
• Subjects: PIGA -- GPI anchor -- Gulf War veterans -- depleted uranium -- in vivo somatic mutations; Mutagenesis -- Periodicals; Molecular genetics -- Periodicals; Mutagenèse -- Périodiques; Mutagenèse chimique -- Périodiques; Mutation -- Périodiques; Maladies de l'environnement -- Périodiques; Génétique moléculaire -- Périodiques; 576.542
• DOI: 10.1002/em.22281
• Languages: English
• ISSNs: 0893-6692

Abstract

Abstract : Fifty Veterans of the first Gulf War in 1991 exposed to depleted uranium (DU) were studied for glycosylphosphatidylinositol‐anchor (GPIa) deficient T‐cell mutants on three occasions during the years 2009, 2011, and 2013. GPIa deficiency was determined in two ways: cloning assays employing aerolysin selection and cytometry using the FLAER reagent for positive staining of GPIa cell surface proteins. Subsequent molecular analyses of deficient isolates recovered from cloning assays (Nicklas JA et al. [2019]: Environ Mol Mutagen) revealed apparent incomplete selection in some cloning assays, necessitating correction of original data to afford a more realistic estimate of GPIa deficient mutant frequency (MF) values. GPIa deficient variant frequencies (VFs) determined by cytometry were determined in the years 2011 and 2013. A positive but nonsignificant association was observed between MF and VF values determined on the same blood samples during 2013. Exposure to DU had no effect on either GPIa deficient MF or VFs. Environ. Mol. Mutagen. 60:494–504, 2019. © 2019 Wiley Periodicals, Inc.