Stoichiometric quantification of spatially dense assemblies with qPAINT. Issue 26 (23rd May 2019)

• Record Type: Journal Article
• Authors: Baker, Matthew A. B.; Nieves, Daniel J.; Hilzenrat, Geva; Berengut, Jonathan F.; Gaus, Katharina; Lee, Lawrence K.
• Is Part Of: Nanoscale. Volume 11:Issue 26(2019)
• Page Start: 12460
• Page End: 12464
• Publication Date: 2019-05-23
• Subjects: Nanoscience -- Periodicals; Nanotechnology -- Periodicals; 620.505
• DOI: 10.1039/c9nr00472f
• Languages: English
• ISSNs: 2040-3364

Abstract

Abstract : DNA qPAINT can be used to quantify the stoichiometry of dense arrays of molecules separated by as little as 3 nm. Abstract : Quantitative PAINT (qPAINT) is a useful method for counting well-separated molecules within nanoscale assemblies. But whether cross-reactivity in densely-packed arrangements perturbs measurements is unknown. Here we establish that qPAINT measurements are robust even when target molecules are separated by as little as 3 nm, sufficiently close that single-stranded DNA binding sites can interact.