T cells in swine completely rearrange immunoglobulin heavy chain genes. (October 2019)

• Record Type: Journal Article
• Authors: Sinkorova, Jana; Stepanova, Katerina; Butler, John E.; Sinkora, Marek
• Is Part Of: Developmental and comparative immunology. Volume 99(2019)
• Publication Date: 2019-10
• Subjects: Porcine immune system -- B cell receptors -- Gene rearrangement -- Lymphocyte repertoire development -- Lymphocyte subpopulations -- Thymus; BCR B cell receptor -- CDR3 complementary determining region 3 -- DG day of gestation -- DH immunoglobulin heavy chain diversity segment -- DJH immunoglobulin incomplete D to J heavy chain rearrangement -- Ig immunoglobulin -- IgH immunoglobulin heavy chain -- IgL immunoglobulin light chain -- JH immunoglobulin heavy chain join segment -- mAb monoclonal antibodies -- MHC-II major histocompatibility class II antigen -- PBS phosphate buffer solution -- PCR polymerase chain reaction -- SWC swine workshop cluster -- TdT terminal deoxynucleotidyl transferase -- VDJH immunoglobulin complete V to D to J heavy chain rearrangement -- VH immunoglobulin heavy chain variable segment; Immunology -- Periodicals; Developmental immunology -- Periodicals; 616.079
• DOI: 10.1016/j.dci.2019.103396
• Languages: English
• ISSNs: 0145-305X

Abstract

Abstract: Porcine thymus contains three independent populations of cells that have rearranged immunoglobulin heavy chain VDJH genes. The first population can be found exclusively in medulla and it consists of existing mature B cells and plasma cells. The second consists of developing B cells characterized by the presence of selected VDJH rearrangement, similar to B cell lymphogenesis in the bone marrow. The third population is entirely unaffected by selection mechanism for productive VDJH rearrangement and represents T lineage cells that rearrange immunoglobulin genes. Transcription of unselected VDJH repertoire is not allowed in T cells. Sequence analysis of unselected VDJH repertoire from T cells also revealed important consequences for B cell lymphogenesis and selection of B cell repertoire. As far as we know, this is the first evidence that some species completely rearrange VDJH genes in T cells. Our results also support the finding that B cells actively develop in the thymus. Graphical abstract: Image 1 Highlights: Porcine thymus contains 3 independent populations of cells with VDJH rearrangements. B cells actively develop in the porcine thymus by similar pathways as in the bone marrow. Mature thymic B cells rearrangements resemble the switched IgG and IgA transcripts. Porcine T cells can completely rearrange VDJH genes. Swine is species in which complete rearrangement of VDJH genes in T cells is allowed.