The potential of biobanked liquid based cytology samples for cervical cancer screening using Raman spectroscopy. Issue 7 (25th March 2019)

• Record Type: Journal Article
• Authors: Traynor, Damien; Duraipandian, Shiyamala; Bhatia, Ramya; Cuschieri, Kate; Martin, Cara M.; O'Leary, John J.; Lyng, Fiona M.
• Is Part Of: Journal of biophotonics. Volume 12:Issue 7(2019)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2019-03-25
• Subjects: biobank -- cervical cancer -- cervical pre‐cancer -- cytology -- Raman spectroscopy -- ThinPrep; Photonics -- Periodicals; Optical materials -- Periodicals; Optics -- Periodicals; Medical instruments and apparatus -- Periodicals; 621.3605
• DOI: 10.1002/jbio.201800377
• Languages: English
• ISSNs: 1864-063X

Abstract

Abstract : Patient samples are unique and often irreplaceable. This allows biobanks to be a valuable source of material. The aim of this study was to assess the ability of Raman spectroscopy to screen for histologically confirmed cases of Cervical Intraepithelial neoplasia (CIN) using biobanked liquid based cytology (LBC) samples. Two temperatures for long term storage were assessed; 80°C and −25°C. The utility of Raman spectroscopy for the detection of CIN was compared for fresh LBC samples and biobanked LBC samples. Two groups of samples were used for the study with one group associated with disease (CIN 3) and the other associated with no disease (cytology negative). The data indicates that samples stored at −80°C are not suitable for assessment by Raman spectroscopy due to a lack of cellular material and the presence of cellular debris. However, the technology can be applied to fresh LBC samples and those stored at −25°C and is, moreover, effective in the discrimination of negative samples from those where CIN 3 has been confirmed. Pooled fresh and biobanked samples are also amenable to the technology and achieve a similar sensitivity and specificity for CIN 3. This study demonstrates that cervical cytology samples stored within biobanks at temperatures that preclude cell lysis can act as a useful resource for Raman spectroscopy and will facilitate research and translational studies in this area. Abstract : This paper investigates Raman spectroscopy for screening confirmed cases of cervical cancer using Long term biobanked samples. Classification of disease was compared using Raman spectra from fresh liquid based cytology samples and biobanked samples. Raman spectroscopy was found to effectively discriminate negative sample disease samples in both fresh and biobanked samples. In addition it was found that biobanked samples could be combined with fresh samples and still achieve a similar sensitivity and specificity.