Chlorococcum turfosum comb. nov. (Volvocales, Chlorophyceae) and visualization of chimeric sequences. Issue 3 (24th January 2019)

• Record Type: Journal Article
• Authors: Nakada, Takashi; Watanabe, Shin; Lewis, Louise A.; Tomita, Masaru
• Is Part Of: Phycological research. Volume 67:Issue 3(2019:Jul.)
• Page Start: 239
• Page End: 243
• Publication Date: 2019-01-24
• Subjects: 18S rRNA -- artificial chimera -- Chlamydomonas acidophila -- Chlorococcum -- clade Moewusinia -- molecular phylogeny; Algae -- Japan -- Periodicals; Algae -- Periodicals; 579.8
• DOI: 10.1111/pre.12365
• Languages: English
• ISSNs: 1322-0829

Abstract

SUMMARY: Parachlorococcum turfosum was previously described based on morphological studies of a culture collection strain originally identified as ' Chlorococcum elkhartiense ' and on an 18S r RNA gene phylogeny of a strain derived from the same original strain but from a different culture collection. However, a subsequently published 18S r RNA gene sequence was inconsistent with the original sequence. Therefore, a new 18S r RNA gene sequence was obtained from the strain from which the holotype was derived. Comparative analyses of the new sequence using the basic local alignment search tool and a newly written Perl code, ChimViewP, which visualizes nucleotide signature shifts characteristic of chimeric sequences, revealed that the sequence used in the original description was of a chimeric nature. Similar analyses also indicated that two published ' Chlamydomonas acidophila ' sequences are chimeric. Phylogenetic analyses excluding all putative chimeras showed that P. turfosum belongs to Chlorococcum, and thus Chlorococcum turfosum comb. nov. is proposed.