MicroRNA-31 Negatively Regulates Interleukin-34 Expression In Vitro. (18th August 2019)

• Record Type: Journal Article
• Authors: Li, Miaomiao; Dong, Yang; Chen, Zhangming; Meng, Lei; Liu, Xingyun; Zhang, Xinhui; Wang, Huimin; Mao, Weijia; Zhang, Jie; Jiang, Zhe; Huang, Ting; Hu, Jie; Luo, Panquan; Korner, Heinrich; Ying, Songcheng; Li, Jun
• Is Part Of: Immunological investigations. Volume 48:Number 6(2019)
• Page Start: 597
• Page End: 607
• Publication Date: 2019-08-18
• Subjects: IL-34 -- MiR-31 -- regulation -- MicroRNA; Immunology -- Periodicals; Immunochemistry -- Periodicals; Cellular immunity -- Periodicals; Communicable diseases -- Periodicals; 616.079
• DOI: 10.1080/08820139.2019.1578230
• Languages: English
• ISSNs: 0882-0139

Abstract

ABSTRACT: Interleukin-34 (IL-34) is a recently discovered cytokine that promotes tissue macrophage maturation and differentiation. We previously found that 1α, 25-Dihydroxyvitamin D3 up-regulated IL-34 expression in SH-SY5Y neural cells. However, whether microRNA regulates IL-34 expression is not completely clear. By using on-line TargetScan and MiRanda software, we found that there was only one conserved microRNA-31 (miR-31) binding site in the 3ʹ untranslated region (3ʹUTR) of IL-34 mRNA. Intriguingly, using qPCR we demonstrated that miR-31 levels were negatively correlated to IL-34 mRNA levels in different cell lines. By examining the effect of miR-31 on IL-34 3ʹ UTR reporter luciferase activity and on IL-34 mRNA and argonaute RISC catalytic component 2 (AGO2) binding, it was found that miR-31 bound directly to IL-34 3ʹUTR and regulated the post-transcriptional expression of IL-34 in MGC-803 cells. Moreover, a miR-31 mimic significantly reduced IL-34 expression levels while a miR-31 inhibitor up-regulated IL-34 expression in KYSE-45 and HT-29 cells. Taken together, these results show that miR-31 negatively regulates IL-34 expression by directly binding to the IL-34 3ʹ UTR in vitro.