Structure analysis and anti-fatigue activity of a polysaccharide from Lepidium meyenii Walp. Issue 17 (2nd September 2019)

• Record Type: Journal Article
• Authors: Tang, Yun; Zhu, Zhen-Yuan; Pan, Li-Chao; Sun, Huiqing; Song, Qiao-Ying; Zhang, Yongmin
• Is Part Of: Natural product research. Volume 33:Issue 17(2019)
• Page Start: 2480
• Page End: 2489
• Publication Date: 2019-09-02
• Subjects: Lepidium meyenii Walp -- polysaccharide -- construction -- anti-fatigue; Natural products -- Periodicals; Natural products -- Research -- Periodicals; Biological Factors -- Periodicals; Biological Products -- Periodicals; 547
• DOI: 10.1080/14786419.2018.1452017
• Languages: English
• ISSNs: 1478-6419

Abstract

Abstract: A polysaccharide was obtained from Lepidium meyenii Walp by hot water extraction and purification by Millipore (100 kD) and Sephadex G-200. The content of polysaccharide was examined to be 89.9% with phenol-sulfuric acid method. Its average molecular weight was estimated to be 2.213 × 10 6 Da by High Performance Gel Permeation Chromatography (HPGPC). Monosaccharide analysis showed that the polysaccharide was composed of arabinose, mannose, glucose and galactose with the molar ratio of 2.134: 1: 2.78: 2.82. After Smith degradation, methylation, infrared spectroscopy and NMR, the primary structure of the polysaccharide was identified. The backbone of the polysaccharide was composed of →4)-β-D-Gal p -(1→ and →4)-α-D-Gal p -(1→, while the branches were comprised of →6)-β-D-Glu p -(1→, →5)- β-D-Ara f -(1→, →3, 6)-α-D-Man p -(1→, →3)-α-D-Gal p -(1→, and α-D-Glu p -(1→. The anti-fatigue effect of the polysaccharide was evaluated using exhaustive swimming test and biochemical indexes. The results indicated the polysaccharide has anti-fatigue effect. Abstract :