Non-obstructive intracellular nanolasers. Issue 1 (December 2018)

• Record Type: Journal Article
• Authors: Fikouras, Alasdair; Schubert, Marcel; Karl, Markus; Kumar, Jothi; Powis, Simon; Di Falco, Andrea; Gather, Malte
• Is Part Of: Nature communications. Volume 9:Issue 1(2018)
• Page Start: 1
• Page End: 7
• Publication Date: 2018-12
• Subjects: Biology -- Periodicals; Physical sciences -- Periodicals; 505
• DOI: 10.1038/s41467-018-07248-0
• Languages: English
• ISSNs: 2041-1723

Abstract

Abstract Molecular dyes, plasmonic nanoparticles and colloidal quantum dots are widely used in biomedical optics. Their operation is usually governed by spontaneous processes, which results in broad spectral features and limited signal-to-noise ratio, thus restricting opportunities for spectral multiplexing and sensing. Lasers provide the ultimate spectral definition and background suppression, and their integration with cells has recently been demonstrated. However, laser size and threshold remain problematic. Here, we report on the design, high-throughput fabrication and intracellular integration of semiconductor nanodisk lasers. By exploiting the large optical gain and high refractive index of GaInP/AlGaInP quantum wells, we obtain lasers with volumes 1000-fold smaller than the eukaryotic nucleus (V laser < 0.1 µm3 ), lasing thresholds 500-fold below the pulse energies typically used in two-photon microscopy (E th ≈ 0.13 pJ), and excellent spectral stability (<50 pm wavelength shift). Multiplexed labeling with these lasers allows cell-tracking through micro-pores, thus providing a powerful tool to study cell migration and cancer invasion. While the sharp emission lines of microlasers are advantageous for cell tagging, their relatively large size can impede cell migration and behavior. Here, Fikouras et al. develop nano-sized lasers that can readily tag and track various types of cells, including through narrow constrictions.