Evolution of six novel ORFs in the plastome of Mankyua chejuense and phylogeny of eusporangiate ferns. Issue 1 (December 2018)

• Record Type: Journal Article
• Authors: Kim, Hyoung; Kim, Ki-Joong
• Is Part Of: Scientific reports. Volume 8:Issue 1(2018)
• Page Start: 1
• Page End: 11
• Publication Date: 2018-12
• Subjects: Natural history -- Research -- Periodicals; Biology -- Research -- Periodicals; Physical sciences -- Research -- Periodicals; Earth sciences -- Research -- Periodicals; Environmental sciences -- Research -- Periodicals; 502.85
• DOI: 10.1038/s41598-018-34825-6
• Languages: English
• ISSNs: 2045-2322

Abstract

Abstract In this paper, three plastomes ofMankyua chejuense, Helminthostachys zeylanica, andBotrychium ternatum in Ophioglossaceae were completely sequenced in order to investigate the plastome evolution and phylogeny of eusporangiate ferns. They were similar to each other in terms of length and the gene orders; however, six unknown open reading frames (ORFs) were found betweenrps4 andtrnL -UAA genes inM. chejuense . Similar sequence regions of six ORFs ofM. chejuense were found at the plastomes ofOphioglossum californicum andH. zeylanica, as well as the mitochondrial genome (mitogenome) ofH. zeylanica, but not inB. ternatum . Interestingly, the translated amino acid sequences of three ORFs were more similar to the proteins of distantly related taxa such as algae and bacteria than they were to proteins in land plants. It is likely that the six ORFs region arose from endosymbiotic gene transfer (EGT) or horizontal gene transfer (HGT), but further study is needed to verify this. Phylogenetic analyses suggested thatMankyua was resolved as the earliest diverging lineage and thatOphioglossum was subsequently diverged in Ophioglossaceae. This result supports why the plastome ofM. chejuense have contained the most ancestral six ORFs in the family.