Efficient immunoaffinity chromatography of lymphocytes directly from whole blood. Issue 1 (December 2018)

• Record Type: Journal Article
• Authors: Mohr, Fabian; Przibilla, Sabine; Leonhardt, Franziska; Stemberger, Christian; Dreher, Stefan; Müller, Thomas R.; Fräßle, Simon P.; Schmidt, Georg P.; Kiene, Marie-Luise; Stadler, Herbert; Busch, Dirk
• Is Part Of: Scientific reports. Volume 8:Issue 1(2018)
• Page Start: 1
• Page End: 6
• Publication Date: 2018-12
• Subjects: Natural history -- Research -- Periodicals; Biology -- Research -- Periodicals; Physical sciences -- Research -- Periodicals; Earth sciences -- Research -- Periodicals; Environmental sciences -- Research -- Periodicals; 502.85
• DOI: 10.1038/s41598-018-34589-z
• Languages: English
• ISSNs: 2045-2322

Abstract

Abstract We show that defined lymphocytes can be rapidly purified by immunoaffinity chromatography starting directly from whole blood. The method relies on low-affinity Fab-fragments attached to a column-matrix combined with the reversible Strep-tag technology. Compared to established cell enrichment protocols, the Strep-tag affinity chromatography of cells is independent of erythrocyte lysis or centrifugation steps, allowing for simple cell-enrichment with good yields, high purities, and excellent functionality of purified cells.